VU Lab M13 – Unknown Identity: Cardiovascular Infection

Before starting this lab, make sure you have completed and understand all of the activities in
the previous labs.  

Lab M13 Activities:

1.  Open the Virtual Unknown Software.

2.  Select and name a new unknown with the subgroup
Cardiovascular 1.

3.  Read through the case and assign the
Gram stain status.

4.  Identify the unknown organism using the following guidelines:

* If the bacteria is Gram negative, start with the IMViC series covered in lab #M9

* If the bacteria is Gram positive, start with whichever of the following tests will eliminate the
most possibilities – OF Glucose, Growth in NaCl, or Blood Hemolysis

* When choosing tests, try to avoid tests that give results of [+] or d since they may not give
accurate results for all samples

* Continue to do tests until only one possible organism remains.  If at any point you eliminate
all possibilities, return to the test that says “Remaining {0}” and redo that test.

* You can start over at any time by choosing “New Unknown”.

5.  If you forget how to do a test, or you want to do a test not previously covered, use the T?
and M? buttons at the top of the lab page for directions.

6.  Identify the unknown bacteria for subgroup
Cardiovascular 1.  Once you identify the
unknown, save your .pdf report to upload to the dropbox.  

7.  Next, repeat the process for a new unknown for subgroup
Cardiovascular 2.

8.  Identify the unknown bacteria for subgroup
Cardiovascular 2.  Once you identify the
unknown, save your .pdf report to upload to the dropbox.  


Answer each of the following questions and upload the answers to the dropbox in a
Word document, along with your two .pdf lab reports.  

1.  Cardiovascular 1 identity: ________________________  Case study # _____________
Cardiovascular 2 identity: __________________________  Case study # _____________

2.  For Cardiovascular 1:  Click on the B? button at the top of the lab page then click on
Reference Books and either the Gram Positive or Gram Negative folder (whichever applies).  
Find the bacteria in the list that matches the “Assigned Unknown” listed on your lab report.  

a.  What symptoms does infection by this bacteria normally cause?  

b.  Describe where this bacteria is normally found in the body and where it causes infection.  

c.  What details listed in the case study give clues to how this infection occurred? (You may
have to think outside the box for this last answer – remember, this program is not just used in
nursing programs, it is also used in vet programs).

3.  For Cardiovascular 2:  Click on the B? button at the top of the lab page then click on
Reference Books and either the Gram Positive or Gram Negative folder (whichever applies).  
Find the bacteria in the list that matches the “Assigned Unknown” listed on your lab report.  

a.  What symptoms does infection by this bacteria normally cause?  

b.  Where is this bacteria typically found?  What type of predisposing factors increase the
chances of infection by this organism?  

c.  What details listed in the case study give clues to how this infection occurred? (You may
have to think outside the box for this last answer – remember, this program is not just used in
nursing programs, it is also used in vet programs).

4.  The cardiovascular system is exposed to bacteria during dental work.  Describe two
precautions dentists take to minimize the risk of infection.

5.  Research the disease "endocarditis".  Explain, in your own words, how bacteria cause
this illness and what specific area of the heart is most impacted.