VU Lab M14 – Unknown Identity: Urinary Tract Infection

Before starting this lab, make sure you have completed and understand all of the activities in
the previous labs.  

Lab M14 Activities:

1.  Open the Virtual Unknown Software.

2.  Select and name a new unknown with the subgroup
Urinary Tract 1.

3.  Read through the case and assign the
Gram stain status.

4.  Identify the unknown organism using the following guidelines:

* If the bacteria is Gram negative, start with the IMViC series covered in lab #M9

* If the bacteria is Gram positive, start with whichever of the following tests will eliminate the
most possibilities – OF Glucose, Growth in NaCl, or Blood Hemolysis

* When choosing tests, try to avoid tests that give results of [+] or d since they may not give
accurate results for all samples

* Continue to do tests until only one possible organism remains.  If at any point you eliminate
all possibilities, return to the test that says “Remaining {0}” and redo that test.

* You can start over at any time by choosing “New Unknown”.

5.  If you forget how to do a test, or you want to do a test not previously covered, use the T?
and M? buttons at the top of the lab page for directions.

6.  Identify the unknown bacteria for subgroup
Urinary Tract 1.  Once you identify the
unknown, save your .pdf report to upload to the dropbox.  

7.  Next, repeat the process for a new unknown for subgroup
Urinary Tract 2.

8.  Identify the unknown bacteria for subgroup
Urinary Tract 2.  Once you identify the
unknown, save your .pdf report to upload to the dropbox.  


Answer each of the following questions and upload the answers to the dropbox in a
Word document, along with your two .pdf lab reports.  

1.  Urinary Tract 1 identity: ________________________  Case study # _____________
Urinary Tract 2 identity: __________________________  Case study # _____________

2.  For Urinary Tract 1:  Click on the B? button at the top of the lab page then click on
Reference Books and either the Gram Positive or Gram Negative folder (whichever applies).  
Find the bacteria in the list that matches the “Assigned Unknown” listed on your lab report.  

a.  What symptoms does infection by this bacteria normally cause?  

b.  Describe why the ability of this bacteria to use urea for energy is beneficial in a urinary tract
infection (please give a complete explanation, in your own words for full credit).  

c.  What details listed in the case study give clues to how this infection occurred? (You may
have to think outside the box for this last answer – remember, this program is not just used in
nursing programs, it is also used in vet programs).

3.  For Urinary Tract 2:  Click on the B? button at the top of the lab page then click on
Reference Books and either the Gram Positive or Gram Negative folder (whichever applies).  
Find the bacteria in the list that matches the “Assigned Unknown” listed on your lab report.  

a.  What symptoms does infection by this bacteria normally cause?  

b.  Explain why fecal bacteria are commonly found causing urinary tract infections.  

c.  What details listed in the case study give clues to how this infection occurred? (You may
have to think outside the box for this last answer – remember, this program is not just used in
nursing programs, it is also used in vet programs).

4.  Research and compare the diseases cystitis and pyelonephritis.  Compare and contrast
their location and symptoms.  (All answers must be in your own words, not copy and paste)

5.  This is your final VU lab this term!! :)  List two things in this program that helped you learn
the class material and two things that could be improved upon.